Maturation of B Cells:

B-cell precursors go through sequential stages of maturation:

• Early B stage
• CD34⁺ CD10^- TdT⁺ cCD79a⁺ CD19⁺
• Pro-B stage
• CD34⁺ CD19⁺ CD10⁺ TdT⁺ CD38⁺⁺ CD20^- cIgM^-
• Pre-B cells Subsets I and II
• Downregulation of CD34 and TdT
• CD34- CD19⁺ CD10⁺ CD38⁺⁺ cIgM⁺ CD20^{heterogeneous}
• Immature B-cells
• CD34^- CD19⁺ CD20⁺ CD38⁺⁺ CD10^dim/- sIgM^low/-
• Mature naïve B-cells
• After expression of light chains
• CD10^- CD19⁺ CD38^+/- CD20⁺ sIgM⁺ sIgD⁺
(Adapted from Porwit and Béné 2018)

Tubes designed to assess B-lymphoblastic leukemia contain makers that allow the distinction of maturation subsets.

This is a bone marrow sample from a young patient with increased numbers of normal B-cell precursors.

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