Learn Flow Cytometry
Welcome to Learn Flow Cytometry.
There are a number of books and other resources that cover the mechanics of flow cytometry and the various markers that are used. This website is meant to complement available resources and aims to provide a practical and comprehensive approach to flow cytometry analysis, addressing issues such as how to recognize patterns and classify normal or aberrant cell populations, how to assess maturation patterns and how to avoid certain pitfalls when analyzing a case.
Unless otherwise specified, the references for the content presented are:
- Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies, by Anna Porwit and Marie Christine Béné, 2018
- WHO classification of Tumours of Haematopoietic and Lymphoid Tissues, Swerdlow et al. 2017
Cases were provided by our collaborators.
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Expand All TopicsBasics of flow cytometry analysis
- Screening Flow Cytometry
- Maturation of B-cells
- Maturation of Myeloid Populations
- How to identify unusual cell populations (basophils, mast cells, etc.) *
Assessment of B-cell populations
- Normal B-cell subsets and reactive changes
- Approach to clonal B-cell populations
- Review of antigens used in the diagnosis of B-cell lymphoproliferative disorders
- CD5+ B-cell neoplasms
- Chronic lymphocytic leukemia/Small lymphocytic lymphoma (CLL/SLL)
- Chronic lymphocytic leukemia/Small lymphocytic lymphoma (CLL/SLL)- Variations
- Mantle cell lymphoma
- Case of interest
- CD10+ B-cell neoplasms
- CD5-CD10- B-cell neoplasms
- Large B-cell lymphomas
- Commonly used molecular ancillary testing for the diagnosis of B-cell lymphoproliferative disorders
Assessment of plasma cells *
T-cells and NK-cells
Dysplasia *
Aberrant T-cell population *
Acute leukemia
- Acute myeloid leukemia
- NOS
- APL (acute promyelocytic leukemia)
- NPM1 mutated
- KMT2A *
- CEBPA
- AML with t(16;16) or inv(16)
- AML with t(8;21)
- B-Lymphoblastic Leukemia
- T-Lymphoblastic Leukemia
- Acute Leukemia of Ambiguous Lineage
- MPAL (2 different populations vs biphenotypic population) *
- Plasma cell neoplasm *
* Content under development
Unusual cases *
Minimal residual disease *
* Indicates content is under development
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Updated: 2023-01-11